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dc.contributor.authorVega Mas, Izargi Aida
dc.contributor.authorCukier, Caroline
dc.contributor.authorColeto Reyes, Inmaculada
dc.contributor.authorGonzález Murua, María del Carmen Begoña
dc.contributor.authorLimami, Anis M.
dc.contributor.authorGonzález Moro, María Begoña
dc.contributor.authorMarino Bilbao, Daniel
dc.identifier.citationScientific Reports 9 : (2019) // Article ID 8925es_ES
dc.description.abstractProper carbon (C) supply is essential for nitrogen (N) assimilation especially when plants are grown under ammonium (NH4+) nutrition. However, how C and N metabolic fluxes adapt to achieve so remains uncertain. In this work, roots of wheat (Triticum aestivum L.) plants grown under exclusive NH4+ or nitrate (NO3-) supply were incubated with isotope-labelled substrates ((NH4+)-N-15, (NO3-)-N-15, or [C-13]Pyruvate) to follow the incorporation of N-15 or C-13 into amino acids and organic acids. Roots of plants adapted to ammonium nutrition presented higher capacity to incorporate both (NH4+)-N-15 and (NO3-)-N-15 into amino acids, thanks to the previous induction of the NH4+ assimilative machinery. The N-15 label was firstly incorporated into [N-15]Gln via glutamine synthetase; ultimately leading to [N-15]Asn accumulation as an optimal NH4+ storage. The provision of [C-13]Pyruvate led to [C-13]Citrate and [C-13] Malate accumulation and to rapid [C-13]2-OG consumption for amino acid synthesis and highlighted the importance of the anaplerotic routes associated to tricarboxylic acid (TCA) cycle. Taken together, our results indicate that root adaptation to ammonium nutrition allowed efficient assimilation of N thanks to the promotion of TCA cycle open flux modes in order to sustain C skeleton availability for effective NH4+ detoxification into amino acids.es_ES
dc.description.sponsorshipThe research leading to these results has received funding from the Basque Government (IT-932-16), the Spanish Government (AGL2015-64582-C3-2-R MINECO/FEDER) and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement number 334019. The authors thank the technical and human support provided by Phytotron Service (SGIker, UPV/EHU).es_ES
dc.publisherNature Publishing Groupes_ES
dc.subjectNAD(H)-dependent glutamate-dehydrogenasees_ES
dc.subjectnitrogen use efficiencyes_ES
dc.subjecttriticum-aestivum L.es_ES
dc.subjectphosphoenolpyruvate carboxylasees_ES
dc.subjectasparagine synthetasees_ES
dc.subjectilluminated leaveses_ES
dc.subjecttomato plantses_ES
dc.subjectgaba shuntes_ES
dc.titleIsotopic labelling reveals the efficient adaptation of wheat root TCA cycle flux modes to match carbon demand under ammonium nutritiones_ES
dc.rights.holderThis article is licensed under a Creative Commons Attribution 4.0 International License. (CC BY 4.0)es_ES
dc.rights.holderAtribución 3.0 España*
dc.contributor.funderEuropean Commission
dc.departamentoesBiología vegetal y ecologíaes_ES
dc.departamentoeuLandaren biologia eta ekologiaes_ES

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This article is licensed under a Creative Commons Attribution 4.0 International License. (CC BY 4.0)
Except where otherwise noted, this item's license is described as This article is licensed under a Creative Commons Attribution 4.0 International License. (CC BY 4.0)