dc.contributor.author | Er, Engin | |
dc.contributor.author | Sánchez Iglesias, Ana | |
dc.contributor.author | Silvestri, Alessandro | |
dc.contributor.author | Arnaiz, Blanca | |
dc.contributor.author | Liz Marzán, Luis Manuel | |
dc.contributor.author | Prato, Maurizio | |
dc.contributor.author | Criado Fernández, Alejandro | |
dc.date.accessioned | 2021-03-23T09:21:44Z | |
dc.date.available | 2021-03-23T09:21:44Z | |
dc.date.issued | 2021-02-24 | |
dc.identifier.citation | ACS Applied Materials & Interfaces 13(7) : 8823-8831 (2021) | es_ES |
dc.identifier.issn | 1944-8244 | |
dc.identifier.issn | 1944-8252 | |
dc.identifier.uri | http://hdl.handle.net/10810/50744 | |
dc.description.abstract | The detection of cancer biomarkers at an early stage of tumor development is vital for effective diagnosis and treatment of cancer. Current diagnostic tools can often detect cancer only when the biomarker levels are already too high, so that the tumors have spread and treatments are less effective. It is urgent therefore to develop highly sensitive assays for the detection of such biomarkers at the lowest possible concentration. In this context, we developed a sandwich immunoassay based on surface-enhanced Raman scattering (SERS) for the ultrasensitive detection of alpha-fetoprotein (AFP), which is typically present in human serum as a biomarker indicative of early stages of hepatocellular carcinoma. In the immunoassay design, molybdenum disulfide (MoS2) modified with a monoclonal antibody was used as a capture probe for AFP. A secondary antibody linked to an SERS-encoded nanoparticle was employed as the Raman signal reporter, that is, the transducer for AFP detection. The sandwich immunocomplex "capture probe/target/SERS tag" was deposited on a silicon wafer and decorated with silver-coated gold nanocubes to increase the density of "hot spots" on the surface of the immunosensor. The developed SERS immunosensor exhibits a wide linear detection range (1 pg mL(-1) to 10 ng mL(-1)) with a limit of detection as low as 0.03 pg mL(-1) toward AFP with good reproducibility (RSD < 6%) and stability. These parameters demonstrate that the proposed immunosensor has the potential to be used as an analytical platform for the detection of early-stage cancer biomarkers in clinical applications | es_ES |
dc.description.sponsorship | E.E. is grateful for a fellowship from the Scientific and Technological Research Council of Turkey (TUBITAK). A.C. and A.S. thank MINECO for their research grant (Juan de la Cierva.Incorporacion/no. IJCI-2016-31113, Juan de la Cierva. Formacion/no. FJC2018-036777-I, respectively). We thank Dr. Judith Langer for support in the interpretation of SERS results. This work was supported by the European Union's Horizon 2020 research and innovation program under Grant Agreements 785219 and 881603 Graphene Flagship. L.M.L.-M. acknowledges funding from the European Research Council (ERC-AdG-2017#787510). M.P., as the recipient of the AXA Bionanotechnology Chair, is grateful to the AXA Research Fund for financial support. This work was performed under the Maria de Maeztu Units of Excellence Program from the Spanish State Research Agency.grant no. MDM-20170720 | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | American Chemical Society | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/IJCI-2016-31113 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MINECO/FJC2018-036777-I | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/785219 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/881603 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/787510 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/es/ | * |
dc.subject | molybdenum disulfide | es_ES |
dc.subject | Au-Ag core-shell nanostructures | es_ES |
dc.subject | immunosensor | es_ES |
dc.subject | surface-enhanced Raman scattering (SERS) | es_ES |
dc.subject | alpha-fetoprotein | es_ES |
dc.title | Metal Nanoparticles/MoS2 Surface-Enhanced Raman Scattering-Based Sandwich Immunoassay for a-Fetoprotein Detection | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY-NC-ND 4.0) | es_ES |
dc.rights.holder | Atribución-NoComercial-SinDerivadas 3.0 España | * |
dc.relation.publisherversion | https://pubs.acs.org/doi/10.1021/acsami.0c22203 | es_ES |
dc.identifier.doi | 10.1021/acsami.0c22203 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Química aplicada | es_ES |
dc.departamentoeu | Kimika aplikatua | es_ES |