dc.contributor.author | Li, Wenhong | |
dc.contributor.author | Sancho Erkizia, Ana ![ORCID](/themes/Mirage2//images/orcid_16x16.png) | |
dc.contributor.author | Chung, Wen-Lu | |
dc.contributor.author | Vinik, Yaron | |
dc.contributor.author | Groll, Jürgen | |
dc.contributor.author | Zick, Yehiel | |
dc.contributor.author | Medalia, Ohad | |
dc.contributor.author | Bershadsky, Alexander D. | |
dc.contributor.author | Geiger, Benjamin | |
dc.date.accessioned | 2021-04-28T08:16:18Z | |
dc.date.available | 2021-04-28T08:16:18Z | |
dc.date.issued | 2021-04-27 | |
dc.identifier.citation | Journal Of Cell Science 134(8) : (2021) // Article ID jcs252221 | es_ES |
dc.identifier.issn | 1477-9137 | |
dc.identifier.uri | http://hdl.handle.net/10810/51217 | |
dc.description.abstract | The mechanisms underlying the cellular response to extracellular matrices (ECM), consisting of multiple adhesive ligands, are still poorly understood. Here we address this topic by monitoring the differential cellular response to two different extracellular adhesion molecules - fibronectin, a major integrin ligand, and galectin-8, a lectin, that binds beta-galactoside residues, as well as to mixtures of the two proteins. Cell spreading on galectin-8 coated substrates results in a larger projected cell area, a more extensive extension of filopodia, yet an inability to form focal adhesions and stress fibers, compared to cell spreading on fibronectin. These differences can be partially reversed by experimental manipulations of small G-proteins of the Rho family and their downstream targets, such as formins, Arp2/3 complex, and Rho kinase. We also show that the physical adhesion of cells to galectin-8 is stronger than the adhesion to fibronectin. Notably, galectin-8 and fibronectin differentially regulate cell spreading and focal adhesion formation, yet they act synergistically to upregulate the number and length of filopodia. The physiological significance of the coherent cellular response to a molecularly complex matrix is discussed | es_ES |
dc.description.sponsorship | B.G. is grateful to the Israel Science Foundation (grant no. 2749/17), to the Minerva Center at the Weizmann Institute ‘Aging, from Physical Materials to Human Tissues’ for their support and to the Jean-Jacques Brunschwig Fund for the Molecular Genetics of Cancer for supporting this research. A.D.B. acknowledge the support from the Singapore Ministry of Education (MOE) Academic Research Fund Tier 2 (grant no. MOE2018-T2-2-138), the National Research Foundation, Prime Minister's Office, Singapore, and the Ministry of Education under the Research Centers of Excellence program through the Mechanobiology Institute, Singapore (grant no. R-714-006-006-271), and Singapore Ministry of Education Academic Research Fund Tier 3 MOE grant no. MOE2016-T3-1-002. A.D.B., B.G. and W.L. acknowledge the support from a Maimonides Israeli–France grant (Israeli Ministry of Science Technology and Space), and EU Marie Skłodowska-Curie Network InCeM (grant no. 642866) at the Weizmann Institute of Science. A.S. and J.G. acknowledge financial support from the European Research Council (ERC; grant no. 617989). W.-L.C. and O.M. acknowledge the ERC-Syg grant (grant no. 810057-HighResCells): Open access funding provided by Weizmann Institute of Science. Deposited in PMC for immediate release | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | The Company of Biologists | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/642866 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/FP7/617989 | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/810057 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by/3.0/es/ | * |
dc.subject | extracellular matrix | es_ES |
dc.subject | filopodia | es_ES |
dc.subject | focal adhesions | es_ES |
dc.subject | lamellipodia | es_ES |
dc.subject | Myosin-II | es_ES |
dc.subject | Rho GTPases | es_ES |
dc.title | Differential Cellular Responses to Adhesive Interactions with Galectin-8 and Fibronectin Coated Substrates | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) | es_ES |
dc.rights.holder | Atribución 3.0 España | * |
dc.relation.publisherversion | https://journals.biologists.com/jcs/article/134/8/jcs252221/237785/Differential-cellular-responses-to-adhesive | es_ES |
dc.identifier.doi | 10.1242/jcs.252221 | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Ingeniería de sistemas y automática | es_ES |
dc.departamentoeu | Sistemen ingeniaritza eta automatika | es_ES |