dc.contributor.author | Gómez Redondo, Marcos | |
dc.contributor.author | Delgado, Sandra | |
dc.contributor.author | Núñez Franco, Reyes | |
dc.contributor.author | Jiménez Oses, Gonzalo | |
dc.contributor.author | Ardá, Ana | |
dc.contributor.author | Jiménez Barbero, Jesús | |
dc.contributor.author | Gimeno, Ana | |
dc.date.accessioned | 2022-01-04T10:50:47Z | |
dc.date.available | 2022-01-04T10:50:47Z | |
dc.date.issued | 2021-06-01 | |
dc.identifier.citation | RSC Chemical Biology 2(3) : 932-941 (2021) | es_ES |
dc.identifier.issn | 2633-0679 | |
dc.identifier.uri | http://hdl.handle.net/10810/54819 | |
dc.description.abstract | The interaction of human galectin-8 and its two separate N-terminal and C-terminal carbohydrate recognition domains (CRD) to their natural ligands has been analysed using a synergistic combination of experimental NMR and ITC methods, and molecular dynamics simulations. Both domains bind the minimal epitopes N-acetyllactosamine (1) and Gal beta 1-3GalNAc (2) in a similar manner. However, the N-terminal and C-terminal domains show exquisite and opposing specificity to bind either Neu5Ac- or Fuc-containing ligands, respectively. Moreover, the addition of the high-affinity ligands specific for one of the CRDs does not make any effect on the binding at the alternative one. Thus, the two CRDs behave independently and may simultaneously target different molecular entities to promote clustering through the generation of supramolecular assemblies. | es_ES |
dc.description.sponsorship | This research was funded by European Research Council for financial support (ERC-2017-AdG, project number 788143RECGLYCANMR). We also thank Agencia Estatal de Investigacio ' n (Spain) for projects RTI2018-094751-B-C21, RTI2018-099592-B-C22 and the Severo Ochoa Excellence Accreditation (SEV-2016-0644) and the Mizutani Foundation for Glycoscience (200077 to GJO). | es_ES |
dc.language.iso | eng | es_ES |
dc.publisher | Royal Society of Chemistry | es_ES |
dc.relation | info:eu-repo/grantAgreement/EC/H2020/788143 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MCIU/RTI2018-099592-B-C22 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MCIU/RTI2018-094751-B-C21 | es_ES |
dc.relation | info:eu-repo/grantAgreement/MICINN/SEV-2016-0644 | es_ES |
dc.rights | info:eu-repo/semantics/openAccess | es_ES |
dc.rights.uri | http://creativecommons.org/licenses/by-nc/3.0/es/ | * |
dc.subject | protein | es_ES |
dc.subject | recognition | es_ES |
dc.subject | affinity | es_ES |
dc.subject | chain | es_ES |
dc.title | The two domains of human galectin-8 bind sialyl- and fucose-containing oligosaccharides in an independent manner. A 3D view by using NMR | es_ES |
dc.type | info:eu-repo/semantics/article | es_ES |
dc.rights.holder | This is an open Access Article. Published on 12 April 2021. Downloaded on 1/4/2022 9:51:30 AM.This article is licensed under aCreative Commons Attribution-NonCommercial 3.0 Unported Licence | es_ES |
dc.rights.holder | Atribución-NoComercial 3.0 España | * |
dc.relation.publisherversion | https://pubs.rsc.org/en/content/articlelanding/2021/CB/D1CB00051A | es_ES |
dc.identifier.doi | 10.1039/d1cb00051a | |
dc.contributor.funder | European Commission | |
dc.departamentoes | Química orgánica II | es_ES |
dc.departamentoeu | Kimika organikoa II | es_ES |